When we sequence BAC library using NGS, how many clones should we mix? When we use 454 to do it, can we mix 6 or 12 clones to sequence a full run? And if we use illumine how many can we mix? Thank you!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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