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modENCODE project aims to provide the biological research community with a comprehensive encyclopedia of genomic functional elements in the model organisms C. elegans and D.melanogaster. Data Coordination Center for modENCODE is conducting a series of webinars with the next one, "Understanding worm and fly chromatin modification data", scheduled on Thursday Dec 1, 12:00 EST (Eastern US time). This online webinar will introduce participants to the data obtained in experimental studies of various histone modification marks and chromatin binding proteins, also showing how to visualize and download these data from the modENCODE web site/FTP. Space is limited so advance registration is necessary. Please email [email protected] to reserve your spot. More details and the schedule are at http://wiki.modencode.org/project/in...ility_webinars
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.Yesterday, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...