When identifying some organisms, e.g. microorganisms in complex communities, amplicon sequencing based on Illumina short reads is insufficient. It has already been widely accepted that longer DNA regions must be sequenced to achieve species- or strain-level resolution. A typical example is the sequencing of the entire 16S rRNA gene, the ITS region, etc.
Oxford Nanopore technology (ONT) is extremely suitable for this approach. This workshop has been designed to provide participants with the skills needed to navigate the metagenomic studies using long reads sequencing by ONT.
Oxford Nanopore technology (ONT) is extremely suitable for this approach. This workshop has been designed to provide participants with the skills needed to navigate the metagenomic studies using long reads sequencing by ONT.
Agenda
- Introduction to ONT Technology
- Introduction to basic operations in linux (not included in online workshop version)
- Fundamentals of metagenomic analysis
- Data manipulation
- Applications in metagenomics
- Practical aspects of ONT sequencing
- Tips and tricks for optimizing ONT workflows in metagenomic studies
- Exploration of metagenomic data analysis tools and techniques
- Databases
- Analysis of real-world metagenomic datasets
- ONT amplicon-based metagenomic analysis: Case Study
- ONT whole-genome metagenomic analysis: Case Study
Who Should Attend?
- Biologists, bioinformaticians, healthcare professionals and laboratory workers engaged in metagenomic analysis or those seeking to enhance their proficiency with ONT technology.
- Suitable also if you want to deepen or revive your knowledge
- ONT Proficiency: You will gain hands-on expertise in analyzing ONT data
- Metagenomic Analysis Techniques: You will learn essential techniques for analyzing metagenomic data
- Interpretation Skills: You will develop the ability to interpret and draw meaningful conclusions from metagenomic datasets
- Best Practices: You will understand the best practices for experimental design and data analysis in metagenomics
- This is a hands-on computer workshop with presentations and practical demonstrations.
- A necessary prerequisite is computer user skills and basic knowledge of molecular biology.
- For online workshop version: Basic knowledge of how to work in terminal is required.
General information
- Language: English
- Printed presentations are provided for in-person events, pdfs for online events (will be emailed to you a few days before the online event commences).
- For in-person events lunches and refreshments are included.
- All tools we use during our events are open-source (free). We never use licenced software unless explicitly stated.
- All participants of our hands-on workshops perform important steps of data analysis themselves, each has their own computer we provide. Using your own computers and / or data files during our data analysis workshops is not possible.
- Time is always CET.
- Lodging, travel, and other incidental expenses (if applicable) are the responsibility of attendees.
- No laboratory experiments are conducted during our events.
- For all our events you will receive a certificate of completion.
About us
This event is organised by SEQme company. We are a DNA sequencing solution provider with laboratories located in Central Europe and serving several hundreds of clients every year.
We organize courses and workshops since 2012. Our team of speakers is comprised of our employees, NGS lab specialists, data analysts and bioinformaticians. For selected events from our portfolio we work together also with our external partners and collaborators, usually bioinformaticians with deep knowledge of particular application-specific pipelines.