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  • richard.nadvornik
    Member
    • Mar 2018
    • 10

    #1

    NGS Data Analysis & Programming Essentials | Workshop, Prague, Czech Republic, 13. - 15.5.2025

    REGISTER HERE


    Some people think they can't handle Next-Generation Sequencing data analysis. They are wrong!


    You can start with zero coding experience. You will get acquainted with various formats of NGS data, learn basic programming skills and understand the background of individual processes used to modify and analyse NGS data. After this three-day workshop, you will not become a bioinformatician, but you will be well on your way to becoming a qualified partner for bioinformaticians in your team. Join our workshop and learn by doing!

    Day 1 - Setting Up Your Environment
    • Introduction to NGS data and its applications
    • Understanding common NGS data formats
    • Getting started with the Linux operating system
    • Navigating the command line and using essential terminal commands
    Day 2 - Ensuring High-Quality Data
    • Using command-line tools to process NGS data
    • Assessing raw data quality through visualization techniques
    • Pre-processing short-read FASTA/FASTQ files with various software tools
    Day 3 - Optimizing Your Dataset
    • Mapping reads to a reference genome
    • Visualizing and evaluating mapping quality
    • Detecting and removing duplicate reads
    • Identifying and handling unmapped reads


    Who Should Attend?

    This hands-on workshop is designed for biologists, healthcare professionals, and lab researchers who want to analyze Next-Generation Sequencing data while gaining confidence in the command line and basic programming concepts.
    • Beginners — No prior coding knowledge needed!
    • Biologists, lab technicians, and healthcare professionals working with sequencing data
    • Anyone who wants to improve their NGS data handling and programming skills
    What will you learn?
    • The fundamentals of NGS data and its formats
    • How to navigate and use the command line efficiently
    • Essential programming skills to process sequencing data
    • Quality control: filtering and cleaning raw NGS reads
    • Read mapping, visualization, and quality assessment
    • Best practices to get a high-quality dataset for further analysis
    Prerequisites
    • This is a hands-on computer workshop with presentations and practical demonstrations.
    • A necessary prerequisite is computer literacy and basic knowledge of molecular biology (DNA, RNA, gene expression, PCR). Knowledge of BASH and the terminal is not required, but is an advantage.

    General information
    • Language: English
    • Printed presentations are provided for in-person events, pdfs for online events (will be emailed to you a few days before the online event commences).
    • For in-person events lunches and refreshments are included.
    • All tools we use during our events are open-source (free). We never use licenced software unless explicitly stated.
    • All participants of our hands-on workshops perform important steps of data analysis themselves, each has their own computer we provide. Using your own computers and / or data files during our data analysis workshops is not possible.
    • Time is always CET.
    • Lodging, travel, and other incidental expenses (if applicable) are the responsibility of attendees.
    • No laboratory experiments are conducted during our events.
    • For all our events you will receive a certificate of completion.
    About us

    This event is organised by SEQme company. We are a DNA sequencing solution provider with laboratories located in Central Europe and serving several hundreds of clients every year.
    We organize courses and workshops since 2012. Our team of speakers is comprised of our employees, NGS lab specialists, data analysts and bioinformaticians. For selected events from our portfolio we work together also with our external partners and collaborators, usually bioinformaticians with deep knowledge of particular application-specific pipelines.
    ​

    REGISTER HERE​
    Richard Nádvorník, PhD., SEQme s.r.o.

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