There appears to be a lot of excitement about the Marco Island conference this year? Are there any Seqanswers members attending who can update the rest of us?
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Sorry. I meant that some information I have (via Agilent reps) tells me that they are about to release an enrichment methodology that has been refined to the degree that it will be available in "kit" format (ie...reagents plus enrichment oligo sets).Originally posted by seqer View PostWhat is kitted? The long oligos for the whole exonome? I think Nimblegen is more ready to release their "kit".
It was a big secret from Agilent reps, that would be annouced at Marco Island.
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Thanks ECO! Has anyone heard anything from agilent at AGBT? If anyone could give a brief summary of AGBT, that would be really awesome!Originally posted by ECO View PostSorry. I meant that some information I have (via Agilent reps) tells me that they are about to release an enrichment methodology that has been refined to the degree that it will be available in "kit" format (ie...reagents plus enrichment oligo sets).
It was a big secret from Agilent reps, that would be annouced at Marco Island.
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Just found the following here:
So it's a solution phase capture array. Figured they would move away from that Church gap-filling/circularization method.“We prepare a labeled derivative of Agilent’s oligo libraries to use for direct capture of target sequences by hybridization. The method is highly multiplexed, with a simple, efficient and inexpensive laboratory process, thus overcoming the sample preparation bottleneck.”
Seems like this will make hybridization time and cost much less than a Nimblegen capture array, especially if they amplify the pool of oligo's after they are cleaved from the chip.
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PacBio Highlight
The highlight of AGBT had to be the first public presentation from Steven Turner at Pacific Biosciences - a fitting way to close a great meeting. Here's more on what young Steve and his PacBio pals are up to:
http://www.bio-itworld.com/BioIT_Con...p;terms=PacBioKevin Davies
Author, The $1,000 Genome
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Kevin Davies
Author, The $1,000 Genome
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