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  • niceday
    Member
    • Apr 2010
    • 68
    #1

    clusters per tile to clusters mm2

    Hello,

    Can someone tell me the conversion factor from cluster per tile to clusters per mm2. It would be very helpful.
    Thanks.
    Tags: None
  • kmcarr
    Senior Member
    • May 2008
    • 1181
    #2
    This varies dependent upon instrument and flow cell version. However currently for the GAIIx, flowcell v4 the size is 0.5437 mm^2 per tile.

    Comment

    • niceday
      Member
      • Apr 2010
      • 68
      #3
      Thanks for that. I'm trying to get a rough idea of the number of sequence reads so I know if we have enough after the 25bp PF stage.

      Comment

      • kmcarr
        Senior Member
        • May 2008
        • 1181
        #4
        Originally posted by niceday View Post
        Thanks for that. I'm trying to get a rough idea of the number of sequence reads so I know if we have enough after the 25bp PF stage.
        The Sequence Analysis Viewer application reports run statistics during a run. It will directly report the number of PF reads per lane once the run reaches cycle 25 and this number will be calculated appropriately for the flow cell in use. This software is available as part of the SCS2.9_RTA1.9 package for the GAIIx. I believe (but am not positive) that it is included in all versions of the control software for the HiSeq.

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