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currently only the 3′ end and up to 3-kb fragments of mRNAs are obtained, which leaves nearly 36% of genes with mRNAs longer than 3 kb that cannot be fully examined.
Random primers for reverse transcription of purified mRNAs will make it possible to recover full-length cDNAs, including 5′ untranslated and transcription start regions for all expressed genes.
Random primers for reverse transcription of purified mRNAs will make it possible to recover full-length cDNAs, including 5′ untranslated and transcription start regions for all expressed genes.