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  • nicedad
    Member
    • May 2011
    • 49

    #1

    SNPs in 454HCDiffs file

    could anyone please explain how to count # of SNPs in 454HCDiffs file?
  • nickloman
    Senior Member
    • Jul 2009
    • 355

    #2
    If memory serves me correctly each SNP has a header line starting with a >, so:

    grep -e "^>" 454HCDiffs.txt | wc -l

    Should do the trick.

    Comment

    • nickloman
      Senior Member
      • Jul 2009
      • 355

      #3
      You should bear in mind that this would count indels as well as SNPs.

      Comment

      • nicedad
        Member
        • May 2011
        • 49

        #4
        many thanks nickloman,
        However it would be thankful if I could count each type separately (SNPs & INDELs).

        regards,

        Comment

        • nickloman
          Senior Member
          • Jul 2009
          • 355

          #5
          OK, well you could do something like this to count SNPs:

          grep -e "^>" 454HCDiffs.txt | awk '($4 ~ /^[ATGC]$/ && $5 ~ /^[ATGC]$/)' | wc -l

          The difference between that and the previous recipe will be the indels.

          Comment

          • nicedad
            Member
            • May 2011
            • 49

            #6
            Thanks nickloman, I really appreciate your help.

            best,

            Comment

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