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I started working on whole genome sequencing and exome sequencing with Illumina HiSeq 2000 and also with 454 sequencer. Can anybody please help me to understand the data analysis of the sequenced reads????
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If you'd like answers to such a question, you should start by posting in the correct forum. Then, explain what you want to do with the reads. You're query can be condensed to "I ran an experiment; how do I analyze it?", and nobody can really help you without some real detail.Originally posted by rd69 View Post -
Anyone remember the days were you just dumped 3 volumes of Maniatis on a student desk and turned around... probably not.Originally posted by rd69 View PostComment
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Moved this to a new thread...and I really encourage the original poster to read the following:
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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