Hi
Newbie here (to both the site and NGS).
We want to resequence introns, exons and promoters of ~8 cancer genes (~500kb total) for ~100 germline samples.
The inclusion of the introns and promoters makes traditional sanger sequencing out of the question.
We're considering NGS (we have access to ion torrent and illumina at our institute, as well as potentially miseq through Bejing Genome Institute) however, the establishment of libraries for 100 samples is providing to be VERY expensive, especially considering we're only going to be using ~500kb worth per sample.
I would appreciate any suggestions or advice
Thank you!
Newbie here (to both the site and NGS).
We want to resequence introns, exons and promoters of ~8 cancer genes (~500kb total) for ~100 germline samples.
The inclusion of the introns and promoters makes traditional sanger sequencing out of the question.
We're considering NGS (we have access to ion torrent and illumina at our institute, as well as potentially miseq through Bejing Genome Institute) however, the establishment of libraries for 100 samples is providing to be VERY expensive, especially considering we're only going to be using ~500kb worth per sample.
I would appreciate any suggestions or advice
Thank you!