Hi Seqanswers
first I say that I dont have more experience in this area.
Now my group is using for resequecing a GaIIX, but we have the possibility of using a Hiseq but is more expensive, my question is. The area to resequence is 1.5mb, with GAIIX have 50x of coverage, but some areas only 5x, You think that using Hiseq would improve coverage of the 5x? I know that I have more reads , but I'm no sure if i would have diferent reads.
We use SureSelectXt and run 84 samples per plate in the GaIIX , the idea is only run 84 samples in the Hiseq, and the same samples , the same preparation.
Thank you in advance
first I say that I dont have more experience in this area.
Now my group is using for resequecing a GaIIX, but we have the possibility of using a Hiseq but is more expensive, my question is. The area to resequence is 1.5mb, with GAIIX have 50x of coverage, but some areas only 5x, You think that using Hiseq would improve coverage of the 5x? I know that I have more reads , but I'm no sure if i would have diferent reads.
We use SureSelectXt and run 84 samples per plate in the GaIIX , the idea is only run 84 samples in the Hiseq, and the same samples , the same preparation.
Thank you in advance
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