Dear all,
I am pretty new to sequencing and have a newby question:
How come the last nucleotides and the first ones are always bad?
I can understand that the last ones are bad because of polymerase not working ok anymore and perhaps other inhibition factors.
But how come that also the first few nucleotides are also bad?
I am pretty new to sequencing and have a newby question:
How come the last nucleotides and the first ones are always bad?
I can understand that the last ones are bad because of polymerase not working ok anymore and perhaps other inhibition factors.
But how come that also the first few nucleotides are also bad?
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