Hi,
Can anyone share the general pipeline to analyze 454, Illumina/Solexa data?
Thanks a lot for sharing
Can anyone share the general pipeline to analyze 454, Illumina/Solexa data?
Thanks a lot for sharing
-
-
Edge seriously.
This is the wrong forum for this question, and you are asking the most basic of questions that really should be sought with a combination of one's own reading papers and research.
Moving to General, edge please check your private message box.
To at least take a stab at answering this question...
The starting point is almost certainly the software provided by the respective manufacturers for mapping. The remainder depends entirely on the scope of the experiment and what your questions are. (ie. SNP calling, tag counting, mutation discovery, etc) All these applications have various specialized workflows.
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment