what is the latest version of bedtools and can you please provide a download? Thanks.
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Let me google that for you.
2.16.2
Go here:
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Bed_intersect -s empty output
Hi,
I try to use bed intersect to intersect two bed files using -s parameter.
a.bed
b.bedHTML Code:chr1 11804 11900 + 2 0 1 0 3 23 0 0 0 0 4 2 1 0 1 15 0 0 0 0 17 3 00 0 1 0 0 1 1 0 1 8 0 1 1 0 4 4 0 5 0 0 0 1 0 0 2 1 00 0 0 3 0 0 0 0 1 0 0 0 0 4 0 0 2 0 0 0 0 9 0 0 3 0 00 0 5 2 0 0 0 0 1 0 3 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 01 0 0 0 0 0 0 0 5 7 2 0 2 2 2 0 chr1 11804 11900 + 2 0 1 0 3 23 0 0 0 0 4 2 1 0 1 15 0 0 0 0 17 3 00 0 1 0 0 1 1 0 1 8 0 1 1 0 4 4 0 5 0 0 0 1 0 0 2 1 00 0 0 3 0 0 0 0 1 0 0 0 0 4 0 0 2 0 0 0 0 9 0 0 3 0 00 0 5 2 0 0 0 0 1 0 3 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 01 0 0 0 0 0 0 0 5 7 2 0 2 2 2 0 chr1 11804 11900 + 2 0 1 0 3 23 0 0 0 0 4 2 1 0 1 15 0 0 0 0 17 3 00 0 1 0 0 1 1 0 1 8 0 1 1 0 4 4 0 5 0 0 0 1 0 0 2 1 00 0 0 3 0 0 0 0 1 0 0 0 0 4 0 0 2 0 0 0 0 9 0 0 3 0 00 0 5 2 0 0 0 0 1 0 3 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 01 0 0 0 0 0 0 0 5 7 2 0 2 2 2 0
If I use -s parameter bed intersect return an empty file, but if I remove the -s parameter it return a non empty file with some strand match.HTML Code:chr1 11373 12727 NR_046018 DDX11L1 + Ex1* chr1 11873 12227 NR_046018 DDX11L1 + Ex1 chr1 12112 13221 NR_046018 DDX11L1 + Ex2* chr1 12227 12612 NR_046018 DDX11L1 + In chr1 12612 12721 NR_046018 DDX11L1 + Ex2
Any idea?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
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