Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Analysing data from multiple platforms

    Hi all,

    Would anyone care to comment on the validity of combining data from multiple platforms for a targeted resequencing project. In particular, I am looking at data from 454 and MiSeq for amplicon sequencing project. I am assuming the important things to look for are base calling quality, depth and coverage. If those are high quality then there should be no problem publishing the combined data sets?

    Thanks

  • #2
    Originally posted by Strider View Post
    Hi all,

    Would anyone care to comment on the validity of combining data from multiple platforms for a targeted resequencing project. In particular, I am looking at data from 454 and MiSeq for amplicon sequencing project. I am assuming the important things to look for are base calling quality, depth and coverage. If those are high quality then there should be no problem publishing the combined data sets?

    Thanks
    In theory you are able to use multiple experimental methods to converge on the real solution. In practice you end up with more errors and more types of errors, because the analysis techniques are not sufficiently advanced to account for the various types of errors and correct each other. Further more the cost per base of doing something like a Hiseq run washes out anything you could get by doing repeated runs with multiple competing technologies.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-27-2024, 06:37 PM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-27-2024, 06:07 PM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    53 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    69 views
    0 likes
    Last Post seqadmin  
    Working...
    X