Hey all. My PI is planning a project with RNA-seq and I'm supposed to be involved. Any tips on a good place to start learning about RNA-seq, specifically mRNA-seq? I'm a total novice here, send me to the real basic stuff if you can. THanks!
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This is a good place to start: http://en.wikibooks.org/wiki/Next_Ge..._%28NGS%29/RNA
Look around on SeqAnswers (specially through the archives and creative searches using the search tool in menu bar). You will find all kinds of information.
When in doubt, feel free to ask the right questions.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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