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Hey all. My PI is planning a project with RNA-seq and I'm supposed to be involved. Any tips on a good place to start learning about RNA-seq, specifically mRNA-seq? I'm a total novice here, send me to the real basic stuff if you can. THanks!
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This is a good place to start: http://en.wikibooks.org/wiki/Next_Ge..._%28NGS%29/RNA
Look around on SeqAnswers (specially through the archives and creative searches using the search tool in menu bar). You will find all kinds of information.
When in doubt, feel free to ask the right questions.
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The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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