Frozen flow cell

[Meliss a]

Has anyone ever used a flow cell that has been frozen. We had a mishap with our refrigerator and my flow cells all froze!
Tech-support advised against using them but I was wondering if anyone has and what the outcome was.

[ScottC]

I haven't tried it, but I was told that the flowcells will delaminate if they're frozen, or even put on ice.

Let us know how it goes if you do try!

[AndreaStevenesen]

Anyone? @Melissa did you found it? or in elsewhere?

[Riva]

Our lab has the experience....>_<. We test run a Miseq flow cell that has been frozen for 3 weeks in its original buffer. We test run the 150 cycles reagent on it and it turns out to be fine in both cluster density and quality.

[leibyj]

We had accidentally frozen incorporation buffer and a nano flow cell for two weeks. Thawed both in water at room temperature and successfully sequenced with a 300 cycle kit.

[simbapapa]

Thank you

We had accidentally frozen incorporation buffer and a nano flow cell for two weeks. Thawed both in water at room temperature and successfully sequenced with a 300 cycle kit.

Just registered to reply - thank you! Glad to know there is a good chance things will work out fine.