Hey guys, I've been following George Church and Shendure's work and had a general question regarding optimal cost-feasibility for synthesizing a lot of oligos.
The next round of experiments we plan will require on the order of 1 million primers so we are considering array based methods as cost or normal synthesis will be prohibitive. Shendure uses agilent arrays that he strips the oligos off of but I have heard from nimblegen representatives that using strong NaOH washes on their custom arrays also strips their probes off to a "certain degree." can anyone confirm successful design and recovery of sufficient oligo [] from a nimblegen array for PCR? This is not a traditional hyb-elute strategy but it would be a non-traditional use of a nimblegen array. They are much cheaper than agilent options so we'd like to go this route if possible. Please let me know if you get a chance. Thanks!
The next round of experiments we plan will require on the order of 1 million primers so we are considering array based methods as cost or normal synthesis will be prohibitive. Shendure uses agilent arrays that he strips the oligos off of but I have heard from nimblegen representatives that using strong NaOH washes on their custom arrays also strips their probes off to a "certain degree." can anyone confirm successful design and recovery of sufficient oligo [] from a nimblegen array for PCR? This is not a traditional hyb-elute strategy but it would be a non-traditional use of a nimblegen array. They are much cheaper than agilent options so we'd like to go this route if possible. Please let me know if you get a chance. Thanks!
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