Hi Everyone ,
Can anyone can please help me with this
I am using shell script piping to from sra to bam file
My individual commands are
fastq-dump SRR203400.sra
bowtie -v 3 -k 2 --sam /raid/references-and-indexes/hg19/bowtie-indexes/hg19 /raid/development/anusha/SRR203400.fastq > /raid/development/anusha/SRR203400.bowtieold.sam
samtools view -b -S SRR203400.bowtieold.sam > SRR203400.bowtieold.bam
I started piping like this
#!/bin/bash
# generating a fastq file using fastq dump
$1=='sraip'
$2=='hg19ref'
fastq-dump sraip |bowtie -v 3 -k 2 --sam hg19ref outputoffastqdump > $3
I am really getting confused how to use next piping . because i am using fastq dump output in bow tie input and bowtie output in sam input
Thanks in advance,
Anusha.Ch
Can anyone can please help me with this
I am using shell script piping to from sra to bam file
My individual commands are
fastq-dump SRR203400.sra
bowtie -v 3 -k 2 --sam /raid/references-and-indexes/hg19/bowtie-indexes/hg19 /raid/development/anusha/SRR203400.fastq > /raid/development/anusha/SRR203400.bowtieold.sam
samtools view -b -S SRR203400.bowtieold.sam > SRR203400.bowtieold.bam
I started piping like this
#!/bin/bash
# generating a fastq file using fastq dump
$1=='sraip'
$2=='hg19ref'
fastq-dump sraip |bowtie -v 3 -k 2 --sam hg19ref outputoffastqdump > $3
I am really getting confused how to use next piping . because i am using fastq dump output in bow tie input and bowtie output in sam input
Thanks in advance,
Anusha.Ch
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