Hi All,
We've been developing an automated process for using the Quant-iT assay kit to do our DNA quants. Since we're using a liquid handler, we have been thinking about aliquoting out the standards which come in tubes into a 96-well plate. These standards are meant to be stored between -28C to 8C. We can either separate them into single-use plates and store at -20C, or we can store larger aliquots into plates and keep them at 4C.
The problem with the former is that we waste some standards because of dead volume. The problem with the latter is that there are evaporation issues with storing at 4C with a typical foil seal. We want to avoid freeze-thawing standards so keeping a larger plate at -20C is not an option.
What do people typically do in this situation, or for long term storage of solutions, etc. at 4C?
We've been developing an automated process for using the Quant-iT assay kit to do our DNA quants. Since we're using a liquid handler, we have been thinking about aliquoting out the standards which come in tubes into a 96-well plate. These standards are meant to be stored between -28C to 8C. We can either separate them into single-use plates and store at -20C, or we can store larger aliquots into plates and keep them at 4C.
The problem with the former is that we waste some standards because of dead volume. The problem with the latter is that there are evaporation issues with storing at 4C with a typical foil seal. We want to avoid freeze-thawing standards so keeping a larger plate at -20C is not an option.
What do people typically do in this situation, or for long term storage of solutions, etc. at 4C?