Hi all,
I am looking for a fast executable that can join read pairs into single pseudo-reads so that I can apply FastQC on the joined sequences instead of performing two independent fastQC runs.
I wonder why fastQC does not offer that option out of the box!!
I could make my own simple perl/python/java code (using chat-gpt) but am sure there is a more performant compiled version somewhere (my best score is ~6sec / million PE150).
Note: the reads are considered non-overlapping, although many likely are, because I want to get the quality check of the full raw read pair.
I do not intend to merge them for further analysis as they would not correspond to real sequences anymore (plenty of tools for merging exist but will force merging).
Any advice is welcome
Cordially
Stephane
-
The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM