Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • hina
    Junior Member
    • Dec 2023
    • 1

    #1

    extract list ids from a vcf file for Fst calculation

    I would like to calculate Fst from the short-paired reads. For that, I did mapping with minimap2.

    ./minimap2 -ax sr ref.fa read1.fq.gz read2.fq.gz > aln.sam

    # Converted to bam file
    samtools view -Sb aln.sam > aln.bam

    # Sorted the bam file
    samtools sort aln.bam > aln_sorted.bam

    # created an index file of sorted bam
    samtools index aln_sorted.bam

    # created an index file of reference
    samtools faidx ref.fa

    extracted the raw variants using freebayes.
    freebayes -f ref.fa aln_sorted.bam > out_var.vcf

    extracted SNPs using vcf tools
    vcftools --vcf out_var.vcf --remove-indels --recode --recode-INFO-all --out snps_only.vcf

    for getting the ids from the vcf file for Fst calculation, I used bcf tools

    bcftools query -l snps_only.vcf

    I got the output​ `unknown`. How to retrieve the list ids? your suggestions would be appreciated.

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
22 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...