Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Plotting % Occupied by % Pass Filter to optimize loading concentration

    Hi everyone,
    I am working in Ancient DNA laboratory, which has the Next Seq 2000 sequencing platform. I tried to optimize the loading Concentration of different runs by visualizing runs in Sequence Analysis Viewer (SAV) and made a Scatter plot for each one to determine if a run was underloaded, optimally loaded, or overloaded. I would appreciate if anyone could answer me about the following:
    1- If the interpretation of the scatter plots of the next seq 2000 platform is the same as the Nova seq platform ( i.e.Optimal loaded: high pf%, high %occupied, underloaded: positive slope, Overloaded: negative or vertical slope )?
    2- I have three plots that have different shapes that I can not interpret (optimal, underloaded, overloaded) the first one of the Human Affinity enriched library (Ancient DNA library), the second one of force enriched library (Ancient DNA library), and the last one is shotgun ancient DNA library, anyone could help me to interpret?
    Attached Files

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 05-14-2024, 07:03 AM
0 responses
25 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-10-2024, 06:35 AM
0 responses
45 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-09-2024, 02:46 PM
0 responses
59 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-07-2024, 06:57 AM
0 responses
46 views
0 likes
Last Post seqadmin  
Working...
X