NGS Data Analysis

[Solaezek]

Good day, All!

Please I need help regarding my DNA-sequence analysis. I have done alignment and variant calling on my DNA sequence, and have my vcf file. I am having problem transposing my vcf file to csv file format to perform some analysis on Rstudio. I will deeply appreciate any expert guidance on this or any resources that could be of help.

Thank you!

[Solaezek]

Hello, everyone!


I did a sequence alignment and SNPs analysis, which was successful. However, I wanted to include the frequency of each detected SNP in my data. Please, how can I calculate the frequency of reads that identify each SNP in my data? I would deeply appreciate any good guidance.

Thank you!