illuminaCOVID seq kit assay

[hend elshqnqery]

I often lose my library during the final pooling and cleanup step.
Could you please share any recommendations?
Has anyone experienced a similar issue with this kit?

Thank you."

[Tcom111]

Hi,

What was your original library concentration?
Did you run QC between the steps?
What is the library size?
For cleanup, which beads and ratio are you using?
Are you using the kit’s beads or using different ones?

[hend elshqnqery]

Hi

the final library cocn. was 0.7
no, i didnot which steps you recommend to check for QC
-the expected size should be 400bp but i didnot get it at all
- for the beads ratio i followed the illumina protocol ( no. of samples* 5* 0.9)
- yes, i used the kit’s beads