Hi, I would like to know if anyone had already performed miRNA seq with ion proton and could give me an idea of the cost of such a project.
Our aim is to identify miRNA that could be biomarkers of parkinson disease.
And we would like to sequence miRNAs found in the blood of about two hundred subjects (including healthy subjects and patients with AL, PD and other brain diseases).
I also would like to know how many subjects can fill on one chip to achieve a good number of reads even for rare miRNA.
Any other sugestions about the workflow to follow, the minimal number of suject required to achieve statistical significance, useful papers or links are welcome.
Sorry if the answers seems obvious to you, this project is part of my master degree and I'm a beginner in the field
.
Our aim is to identify miRNA that could be biomarkers of parkinson disease.
And we would like to sequence miRNAs found in the blood of about two hundred subjects (including healthy subjects and patients with AL, PD and other brain diseases).
I also would like to know how many subjects can fill on one chip to achieve a good number of reads even for rare miRNA.
Any other sugestions about the workflow to follow, the minimal number of suject required to achieve statistical significance, useful papers or links are welcome.
Sorry if the answers seems obvious to you, this project is part of my master degree and I'm a beginner in the field
.