Hello everyone,
I am constructing a library with ECOP15I. The protocol says efficient cleavage of this enzyme requires the presence of two inversely oriented recognition sites. A head to head orientation is preferred.
Unfortunately, mine is tail to tail direction and the digestion efficiency is low and unstable. I am wondering if there any trick to increase the digestion efficiency? It is too late to change the orientation now.
Thanks a lot!
I am constructing a library with ECOP15I. The protocol says efficient cleavage of this enzyme requires the presence of two inversely oriented recognition sites. A head to head orientation is preferred.
Unfortunately, mine is tail to tail direction and the digestion efficiency is low and unstable. I am wondering if there any trick to increase the digestion efficiency? It is too late to change the orientation now.
Thanks a lot!