MaSurCa Assembler Coverage.
Hi everybody, im working with a De NOVO plant assembly and i used two differents Assemblers, Mira and Masurca, i got a problem with how to know the real coverage in the MaSurCa analisys, because Masurca made super reads with the reads for make the assembly genome and like the literature say, this one is between 2.X an 4.X for a high coverage Illumina sequencing. My question is if somebody how to calculate the real coverage in the MaSurCa genome assembly?.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM