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For any one familiar with RNA-Seq data from TCGA. Are there any control samples included in level3 data. From what I understand from the sample identifier (TCGA-06-0673-11) the last number 11 is for normal tissue and I can't find any samples for RNA-seq.
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Hi Shahenda,
Yes there are a number of normal-adjacent samples available for the RNA-Seq. In fact, you can freely browse and analyze the RNA-SeqV2 (level 3) gene-level data in GenePool by taking advantage of the growing genomics reference library available to everyone. It also includes sample-level metadata curated by folks at Station X to make it easy to find the samples you are interested in analyzing.
Incidentally, we've also included the isoform-, splice-junction-, and exon-level counts in GenePool's premium content.
If you're interested in learning more, please check out GenePool's growing genomics library, check out the following threads:
Please feel free to contact us directly if you have any questions!
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Last edited by GenePool; 11-23-2014, 10:42 PM.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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