If you want to know which sequences (transcripts/unigenes/protein) belong to which biological function/gene ontology term with Blast2GO you can use the "Combined Graph Function" and then export the graph as .txt file (from the side-panel). This gives you a spreadsheet with a list of all sequence IDs for each GO terms (one GO per row) for all GO levels and categories. This means direct and indirect functional annotations for MF, CC and BP.
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
Comment