Hi,
Can someone please tell me what is an "Adapter sequence" and what is its purpose?
Thanks
Can someone please tell me what is an "Adapter sequence" and what is its purpose?
Thanks
-
-
These are any short piece of DNA of known sequence that one adds to the ends of their unknown DNA of interest, usually for the purpose of eventually placing a sequencing primer there. -
Hi,Originally posted by ECO View Post
I have illumina multiplex paired end data of 75bp. The insert size 300-400bp including primer and adapter sequences. I want to check whether my data contains adapter sequence or not for that i used SSAAHA2 and the result file shows matched sequences at different positions like 66th bp to 40th bp, 75th bp to 60th bp. But mostly from the right side of the read.
I want to know can we adapter sequences on both sides of illumina reads.
What if we have 15 bases of adapter sequence out of 20 bases on the right side like from 50th to 65th bp? and then we have other sequence as well that makes up readlength 75bp. Will that be adapter sequence or not?~Adnan~Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment