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greeshma

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Join Date: Jul 2014

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illumina paired end chemistry

07-14-2014, 03:29 AM

Hi,
Does anyone know why we use AT2 and HP3 in PE chemistry? AT2 is formamide and HP3 is NaOH. Both are denaturing agents.They why we do not use AT2 in index reading?? what makes the difference?
thank you
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