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  • Strangelove
    Junior Member
    • Sep 2014
    • 5

    #1

    NGS assignment (a university course)

    HI all, i wasn't able to find a proper place to post this - so.. here it goes

    I have an assignment that requires a complete detailed description of how to establish a new model organism by doing so i have to design a Next Generation Sequencing strategy for the whole chromosomal genome assembly and expression pattern of the given organism. i have to describe the theory of choice related to sequencing technology, sequencing profile/libraries, assembly method and annotation of genomes and transcriptomes.

    it´s a three page assignment, any recommendation regarding choice of strategy and explanatory literature would be greatly appreciated.. well any help at all is appreciated
  • SNPsaurus
    Registered Vendor
    • May 2013
    • 525

    #2
    Has the course given you any information about this? It seems like a hard topic even as the culmination of an intensive course in the topic.
    Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

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    • mastal
      Senior Member
      • Mar 2009
      • 666

      #3
      Find a paper in somewhere like Nature where they report the genome sequence of a model organism, go through the methods in detail and try to understand how and why they used the methods they did.

      Comment

      • Strangelove
        Junior Member
        • Sep 2014
        • 5

        #4
        i am still looking for any explanatory literature, if anyone has anything that they would recommend please post the name of the article or other source
        - thanks

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