Kapa HiFi Polymerase strand displacement

[qwertz]

Hi all,

does anyone know about literature evidence or product sheets that provide information on the strand displacement activity of the Kapa HiFi polymerase?

I am working on optimising my assay and I am looking for a processive polymerase with very little strand displacement ability. I tried to look it up in datasheets, protocols, etc. but have not been able to find concrete information on that. It would be great if someone knows more about that...

Cheers

[austinso]

Generally speaking, high processivity is related to high strand displacement activity, so you won't find a polymerase that fits your requirements. From what I recall, the KAPA enzyme is an evolved Pfx/KOD enzyme and has very strong strand displacement activity and processivity.

There was a review in TiBt that summarized the biochemical properties of various polymerases:

http://www.cell.com/trends/biotechnology/abstract/S0167-7799(04)00058-7?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0167779904000587%3Fshowall%3Dtrue

But I personally think that the NEB site is pretty good:

https://www.neb.com/tools-and-resources/selection-charts/dna-polymerase-selection-chart

[btownshend]

Kapa does not have strand displacement activity

This is a little late for the above discussion, but just in case others search and find this thread... according to Kapa Tech Support, it does NOT have any strand displacement activity.

[petervangalen]

KAPA HiFi HotStart polymerase does not have strand displacement activity

I can confirm the message above, KAPA tech support was very clear about this.

[Tahakwl]

You need a little bit more effort to identify the things properly