Trouble with Egel Size Select

[Winnie]

Hi Everyone,

I am trying to isolate a band in my DNA extraction since on a regular agarose gel, we see two bands and we only want to keep one band since the other is RNA. I am having so much trouble using the Egel. The DNA that I have collected from the wells, show up on the nanodrop to be around 80-150 ng/ul but when I try to run this on a regular gel, I get no bands. I first loaded 2 ul of the sample + 1 ul of 6X loading dye, then the second time with 5 ul of DNA + 1 ul loading dye, still nothing...The original DNA sample pre-size selection shows up just fine on my gel... This is really frustrating me and I'm hoping someone could help me troubleshoot this.

[nucacidhunter]

1- RNase A can be used to remove RNA from DNA without using a gel.
2- NanoDrop quantifies nucleotide regardless of structure (monomer, DNA, RNA, single or double stranded).
3- It is more likely that your collection from E-Gel is not the target band.