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  • Livi81
    Member
    • Apr 2010
    • 22

    #1

    co-factor motif co-ordinates

    Hi all,

    I'll cut right to the chase...I want to identify regions within a chip seq data set that contain a potential co-factor motif. There seems to be lots of programs to indentify motifs within sequences but they don't tell you in which peak sites they occur.

    I want to isolate the peaks occupied by my chip TF and the potential co-factor only so that I can look and see if genes specifically associated with those peaks show similar expression ie. they're potentially co-regulated.

    I originally used the seqpos motif tool in cistrome to look for potential overrepresented motifs in chip seq data to look for potential co-factors. I then tried using 'Screen all motifs' - 'Given a motif, find all regions that match the motif ' but I can't get it to work. I keep getting an error, does anyone know of another program that can do this?

    Many thanks

    Livi
  • Livi81
    Member
    • Apr 2010
    • 22

    #2
    Problem solved, thanks anyway!

    Comment

    • priya
      Member
      • Apr 2013
      • 57

      #3
      Cofactor analysis

      Hi Livi,
      Currently, I am trying to identify the same as you mentioned. May I know how you solved it ?

      Originally posted by Livi81 View Post
      Hi all,

      I'll cut right to the chase...I want to identify regions within a chip seq data set that contain a potential co-factor motif. There seems to be lots of programs to indentify motifs within sequences but they don't tell you in which peak sites they occur.

      I want to isolate the peaks occupied by my chip TF and the potential co-factor only so that I can look and see if genes specifically associated with those peaks show similar expression ie. they're potentially co-regulated.

      I originally used the seqpos motif tool in cistrome to look for potential overrepresented motifs in chip seq data to look for potential co-factors. I then tried using 'Screen all motifs' - 'Given a motif, find all regions that match the motif ' but I can't get it to work. I keep getting an error, does anyone know of another program that can do this?

      Many thanks

      Livi

      Comment

      • Livi81
        Member
        • Apr 2010
        • 22

        #4
        http://opossum.cisreg.ca.

        Hi,
        Yes I did solve it.
        I used a program developed by some local colleagues. I found it very informative and it has an easy to use interface. I hope you find it useful too.

        [URL="http://www.ncbi.nlm.nih.gov/pubmed/22973536"]

        Best wishes

        Livi

        Comment

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