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  • skbrimer
    Member
    • Mar 2014
    • 55

    Frustrations and ventings

    Hi All,

    Socrates believed that true enlightenment was the knowledge that you actually know nothing; so from that definition I must be extremely enlightened.

    Is there a point in your respective careers that you start feeling like you have a handle of the tools and knowledge of this field? I feel like I have huge holes in my knowledge and I am reading everything I can get my hands on and devouring it. I know I will get there. The minimum I want to be able to at least feel comfortable with my work so that if I give it to my boss he doesn't look like a fool in front of everyone.

    I'm going to stop here, since I'm just starting to really ramble on and on.

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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