Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • JdevSEQnoob
    Junior Member
    • Sep 2015
    • 4

    #1

    I Have Variants and positions and need to make a GTF file. Help

    Hi,
    I'm very new to this and I'm working on my first project. I have a list of Variants and their location. I have been tasked with creating a GTF file of this data and then seeing where the data points land. I have searched and have a wealth of knowledge on what makes up the GTF file, why its used, blah blah. But I Still have no idea how to convert the limited amount of data (3 columns) of data that i have into the larger file format of GTF. Please advise. It'd be MUCH appreciated!

    The goal of this piece of the project is to use the coordinates of Variants to find their location in the annotation and then visualize it.
    Last edited by JdevSEQnoob; 09-25-2015, 03:34 AM.
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Just skip making a GTF and use the VCF file that you presumably already have. You can directly use a VCF file with IGV for visualization or things like bedtools for whatever sorts of intersects you might find useful. It's quite likely that many of the tools that you want to use already support the format you have.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    23 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-13-2026, 09:56 AM
    0 responses
    24 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-09-2026, 11:14 AM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Working...