Validation of NGS data

[Odet]

Dear All,

We performed RNA-Seq analysis and differential expression analysis and identified some potential genes. Now we want to validate and proof the function of these genes. We would like to try Virus induces genes silencing (VIGS), to knock out the genes but the problem is that our plant is recalcitrant again agroinfiltration. Are there any other possibilities to validate genes in a non-model plant?

Hoping for a positive replay asap

Thanks!

[Ancestro]

Have you tried with protoplast transcient expression?

http://www.ncbi.nlm.nih.gov/pubmed/16739582