I have two RNA ssRNA oligos that I have annealed into a dsRNA duplex. The concentration of the two ssRNAs were 10uM. What is then the concentration of the dsRNA duplex?
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Really?
If you understand the definition of molarity, then the answer should be obvious (hint: consider the units for molarity). -
If you used equal volume of each oligo at 10 uM (without addition of any buffer), then the concentration of duplex would be 5 uM.Comment
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Nucacidhunter, I was trying to teach a man how to fish...Comment
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Hi HESmith
I understand your approach and agree with you. I just gave a fish for time being.Comment
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Actually it could be 5uM or 10uM depending on what the OP meant by "The concentration of the two ssRNAs were 10uM."
It could mean that the + oligo was at 10uM and the - oligo was at 10uM and an equal amount of each was added to a tube to be annealed. Then the answer is 5uM.
It could mean that the solution of the tube containing the + and - oligo was at 10uM for each. That is 20uM ss-oligo concentration. Then the concentration is 10uM of duplex after they are annealed.
If you think the latter interpretation is ridiculous then what is the concentration of dATP in a 100mM dNTP solution?
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PhillipComment
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I agree with interpretations. To make it clear (I hope) that I assumed #1, my post was more than 3 characters.Originally posted by pmiguel View PostComment
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