I have two RNA ssRNA oligos that I have annealed into a dsRNA duplex. The concentration of the two ssRNAs were 10uM. What is then the concentration of the dsRNA duplex?
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Actually it could be 5uM or 10uM depending on what the OP meant by "The concentration of the two ssRNAs were 10uM."
It could mean that the + oligo was at 10uM and the - oligo was at 10uM and an equal amount of each was added to a tube to be annealed. Then the answer is 5uM.
It could mean that the solution of the tube containing the + and - oligo was at 10uM for each. That is 20uM ss-oligo concentration. Then the concentration is 10uM of duplex after they are annealed.
If you think the latter interpretation is ridiculous then what is the concentration of dATP in a 100mM dNTP solution?
--
Phillip
Comment
-
Originally posted by pmiguel View PostActually it could be 5uM or 10uM depending on what the OP meant by "The concentration of the two ssRNAs were 10uM."
It could mean that the + oligo was at 10uM and the - oligo was at 10uM and an equal amount of each was added to a tube to be annealed. Then the answer is 5uM.
It could mean that the solution of the tube containing the + and - oligo was at 10uM for each. That is 20uM ss-oligo concentration. Then the concentration is 10uM of duplex after they are annealed.
If you think the latter interpretation is ridiculous then what is the concentration of dATP in a 100mM dNTP solution?
--
Phillip
Comment
Latest Articles
Collapse
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Today, 08:06 AM
|
0 responses
11 views
0 likes
|
Last Post
by seqadmin
Today, 08:06 AM
|
||
Started by seqadmin, 04-30-2024, 12:17 PM
|
0 responses
14 views
0 likes
|
Last Post
by seqadmin
04-30-2024, 12:17 PM
|
||
Started by seqadmin, 04-29-2024, 10:49 AM
|
0 responses
19 views
0 likes
|
Last Post
by seqadmin
04-29-2024, 10:49 AM
|
||
Started by seqadmin, 04-25-2024, 11:49 AM
|
0 responses
26 views
0 likes
|
Last Post
by seqadmin
04-25-2024, 11:49 AM
|
Comment