Originally posted by SNPsaurus
View Post
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
oh that' a good idea really! Thanks. I was thinking about fragmentase or some ZnCl2 digestion or so, but wanted to keep it simple.
Leave a comment:
-
Thanks SNPsaurus for reminding Salmon DNA. The product size varies from vendor to vendor. ThermoFisher product size is ≤2,000 bp, Trevigen is 80-500 bp and Agilent one is 300-2000 bp. Other suppliers might have different size range.
Leave a comment:
-
Many places sell sheared salmon sperm DNA as a blocking reagent. I'm not totally sure the size range is in the range you desire, but it might be!
Leave a comment:
-
You cam make your own fragmented DNA by using a cheap source of DNA and 6 or 4 cutter or combination of restriction enzymes. Other option is using NEB Fragmentase product: https://www.neb.com/products/m0348-n...na-fragmentase
I do not think there is a high demand for fragmented DNA, so I never have heard of it. A commercial option is buying SageScience control DNA for Pippin systems. They are restriction fragments and you would need a clean up to remove their loading buffer before using for size selection experiment. http://www.sagescience.com/product-s...-prep-support/
Leave a comment:
-
Buy sheared DNA
Hi all,
I don't have Covaris, and need some sheared control DNA. Ideally, broad range, so I can size select. Anybody knows commercially available sheared DNA (available in EU)?
Thank you.Tags: None
Latest Articles
Collapse
-
by seqadmin
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
Channel: Articles
07-08-2024, 03:19 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 07-25-2024, 06:46 AM
|
0 responses
9 views
0 likes
|
Last Post
by seqadmin
07-25-2024, 06:46 AM
|
||
Started by seqadmin, 07-24-2024, 11:09 AM
|
0 responses
28 views
0 likes
|
Last Post
by seqadmin
07-24-2024, 11:09 AM
|
||
Started by seqadmin, 07-19-2024, 07:20 AM
|
0 responses
161 views
0 likes
|
Last Post
by seqadmin
07-19-2024, 07:20 AM
|
||
Started by seqadmin, 07-16-2024, 05:49 AM
|
0 responses
127 views
0 likes
|
Last Post
by seqadmin
07-16-2024, 05:49 AM
|
Leave a comment: