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  • Buy sheared DNA

    Hi all,

    I don't have Covaris, and need some sheared control DNA. Ideally, broad range, so I can size select. Anybody knows commercially available sheared DNA (available in EU)?

    Thank you.

  • #2
    You cam make your own fragmented DNA by using a cheap source of DNA and 6 or 4 cutter or combination of restriction enzymes. Other option is using NEB Fragmentase product: https://www.neb.com/products/m0348-n...na-fragmentase

    I do not think there is a high demand for fragmented DNA, so I never have heard of it. A commercial option is buying SageScience control DNA for Pippin systems. They are restriction fragments and you would need a clean up to remove their loading buffer before using for size selection experiment. http://www.sagescience.com/product-s...-prep-support/

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    • #3
      Many places sell sheared salmon sperm DNA as a blocking reagent. I'm not totally sure the size range is in the range you desire, but it might be!
      Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

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      • #4
        Thanks SNPsaurus for reminding Salmon DNA. The product size varies from vendor to vendor. ThermoFisher product size is ≤2,000 bp, Trevigen is 80-500 bp and Agilent one is 300-2000 bp. Other suppliers might have different size range.

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        • #5
          oh that' a good idea really! Thanks. I was thinking about fragmentase or some ZnCl2 digestion or so, but wanted to keep it simple.

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          • #6
            Originally posted by SNPsaurus View Post
            Many places sell sheared salmon sperm DNA as a blocking reagent. I'm not totally sure the size range is in the range you desire, but it might be!
            Yes this is true
            SEO Expert Kerala

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