Barcode of plants

[Fonz]

Dear All,

part of my work will be a study on the cpDNA for barcoding of plants, I need a variable region within conserved primers. I think I might look for a conserved gene who presents (in the nucleotide sequence) an intron in within the active site of the protein... does anyone know a chloroplast gene who responds to my requests?

cheers

Alfonso

[Fonz]

cpDNA extraction

dear all, I have one more question, I do not have in the labs here the instruments and the expertize to carry a cpDNA extraction (it seems that an ultracentrifuge is needes according to the protocol of jansens et al 2005). So I should send my samples to someone who extract cpDNA as a routine work. Could you give me any advice?
Sincerely
Fonz