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  • nouba
    Junior Member
    • Mar 2014
    • 5

    #1

    M13 primer confusion!

    Hi!
    I hope someone can help me with this new found interest, DNA sequencing!
    I have heard about M13 primer, but I am not sure of what it is or how/when to use it?? Can someone please help me or guide me?
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250

    #2
    M13 primer often is used to sequence or amplify DNA insert from vectors which have M13 primer sequence. This way one can sequence a cloned DNA fragment without knowing the insert sequences.

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