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  • DNA Sorcerer
    Member
    • Mar 2010
    • 24

    #1

    Sequencing BAC clones with NextGen

    Is there any drawbacks of using Illumina or Solid to sequence BAC clones?

    A company quoted about 10K to sequence one BAC clone (about 140Kb) using the conventional way (shotgun-sanger-assembly), but I thought that I could multiplex 12 different clones in a single Illumina lane and get them all done by half of that money. Am I missing something?

    Cheers
    Hi there
  • ECO
    --Site Admin--
    • Oct 2007
    • 1360

    #2
    Are you anticipating rearrangements or variable repetitive elements that may make assembly difficult? Otherwise it's almost certainly more cost-effective.

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