Has anybody had problems with getting lower than expected DNA concentrations for your normalised amplicons using the SequalPrep Normalisation kit?
According to their protocol, the expected DNA concentration is 1-2ng/ul when using 20ul elution volume. My group is consistently getting below this (0.2-0.8ng/ul). As suggested by the protocol, the starting material we used is >250 ng amplicon per well.
Thanks,
Katherine
According to their protocol, the expected DNA concentration is 1-2ng/ul when using 20ul elution volume. My group is consistently getting below this (0.2-0.8ng/ul). As suggested by the protocol, the starting material we used is >250 ng amplicon per well.
Thanks,
Katherine
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