Hello all,
I am wondering if anyone out there has discovered/created a standardized method for library quantification and quality control...
15 results in 0.0035 seconds.
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Looking for a standardized method of library quantification and quality control
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MiSeq still underclustered at 18 pM
Hello dear people,
We are doing some whole genome sequencing on a plant genome using the MiSeq with v3 reagents for 2x250 paired-end. Library size... -
Quantitation of Nextera XT library
I have searched the forum without success so apologies in advance if I have missed this elsewhere.
We are sequencing pooled Nextera XT... -
RAD-Seq Library Loading Concentration?
Hello,
Would I be correct in assuming that RAD-Seq libraries should be treated as if they were low-diversity amplicon libraries (i.e.... -
NexteraXT Bionalayzer - undertagmentation?
Hi,
I am using the NexteraXT kit to prep mtDNA. I just did a test prep of some samples and had them run on a HS DNA Chip on the Bioanalyzer... -
Low Concentration Library
Hi,
I made libraries using Colon and Lung Cancer Research Panel and quantify them using qPCR. I was wondering why some of the libraries... -
SequalPrep Normalisation kit low DNA concentration
Has anybody had problems with getting lower than expected DNA concentrations for your normalised amplicons using the SequalPrep Normalisation kit? ... -
ddRAD/GBS lib conc on HiSeq 3000/4000 ?
Hi all,
Has anybody any advice on the "best" concentration (pM) to load a ddRAD or GBS libraries on a HiSeq 3000/4000 ? ddRAD... -
Low concentration and large size of Trusight One library
Hi!
I encountered two problems while preparing Illumina's Trusight One library using their guide. I'm following the protocol carefully... -
Low concentration in TruSight One library preparation
Hi!
I encountered two problems while preparing Illumina's Trusight One library using their guide. I'm following the protocol carefully... -
16s updated NextSeq500 library prep for low concentration ok for MiSeq ?
Hi all, anybody has experience with using a 0.5 nM 16s library concentration (instead of 2nM) on the MiSeq ? The NextSeq500 has this protocol (updated... -
library concentrations variations
Hi, everyone,
I made four libraries this week. But today when I ran a DNA gel, I found that the concentrations (DNA band intensity) of... -
Nextera DNA library - low yield?
I just ran the Nextera DNA sample prep for the first time, following the protocol with the exception of using a Zymo column instead of the spin plate.... -
MiSeq cluster generation problems
Hi all,
I'm running a library of synthetic amplicons (~175-250bp), and reading it out with custom primers on the MiSeq. I'm having a lot... -
library concentrations at various levels (RNA seq)
Hi,
I am interested in some library preparation details of RNA seq.
Lets assume you deal with GA or HISeq.
I am just...
