I have what is probably a stupid question, but I'm really not sure what to do. How do people usually use distance to TSS when designing primers for ChIP-qPCR validation? I was planning on using UCSC genome browser, but I'm not sure whether distance to TSS factors in the 5'UTR exon when trying to pull the sequence for a gene. For example, one of the genes that came up in a ChIP-Seq I did was Nhe1, and the distance to TSS is -17. Is that -17 with respect to the atg (which would include the 5'utr sequence), or is it -17 in the 'Promoter/Upstream' sequence that you get if you check the box in the genomic sequence? They aren't the same for most of the genes that I've checked so far. Any help would be greatly appreciated.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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