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Centromere (just wondering)
I understand that we can either fractionate RNA or cDNA so poly A library is not a necessity for RNA-seq. However, would fractionated RNA alone serve to give a decent coverage?
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PolyA selection allows for selection of mRNA. Total RNA from a cell/tissue contains more than 98% of ribosomal RNA sequences and therefore it would be wise to do either polyA selection or remove ribosomal RNA by other means. If not done, you end up having 80% of your reads mappings to ribosomes.Post-doctoral Fellow
John Curtin School of Medical Research
Australian National University, Canberra, ACT, Australia
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
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Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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